[1]刘慧芳 蒋俊俊 杨全略 罗朝莲 梁浩 叶力*.瘦素对HIV-1病毒复制及TLR3/TRIF通路的影响▲[J].内科,2019,14(02):129-132.[doi:10.16121/j.cnki.cn45-1347/r.2019.02.02]
 LIU Huifang,JIANG Junjun,YANG Quanlue,et al.Effects of leptin on HIV-1 virus replication and TLR3/TRIF pathway[J].Internal Medicine of China,2019,14(02):129-132.[doi:10.16121/j.cnki.cn45-1347/r.2019.02.02]
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瘦素对HIV-1病毒复制及TLR3/TRIF通路的影响▲()
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《内科》[ISSN:1006-6977/CN:61-1281/TN]

卷:
14卷
期数:
2019年02
页码:
129-132
栏目:
论著
出版日期:
2019-04-30

文章信息/Info

Title:
Effects of leptin on HIV-1 virus replication and TLR3/TRIF pathway
文章编号:
1673-7768(2019)02-0129-04
作者:
刘慧芳12 蒋俊俊23 杨全略23 罗朝莲12 梁浩234 叶力23*
1 广西医科大学广西生物医药协同创新中心,2 广西艾滋病防治研究重点实验室,3 广西医科大学公共卫生学院,4 广西医科大学生命科学研究院,广西南宁市530021
Author(s):
LIU Huifang12 JIANG Junjun23 YANG Quanlue23 LUO Chaolian12 LIANG Hao234 YE Li23
(1 Guangxi Collaborative Innovation Center for Biomedicine of Guangxi Medical University; 2 Guangxi Key Laboratory of AIDS Prevention and Treatment; 3 School of Public Health, Guangxi Medical University; 4 Life Sciences Institute, Guangxi Medical University; Nanning 530021, China
关键词:
瘦素HIVMT2细胞TLR3TRIF
Keywords:
Leptin HIV MT2 cells TLR3 TRIF
分类号:
R 512.91
DOI:
10.16121/j.cnki.cn45-1347/r.2019.02.02
文献标志码:
A
摘要:
目的了解瘦素对HIV-1病毒在MT2细胞中复制以及对TLR3/TRIF通路相关因子的影响。方法体外培养MT2细胞,将处于对数生长期的MT2细胞分为对照组和实验组。实验组更换用1 μg/mL瘦素培养基,对照组更换用常规培养基,继续培养24 h;将实验组和对照组细胞分别感染HIV-1 3B病毒,继续培养72 h。采用酶联免疫法(ELISA)测定细胞培养上清液中的HIV-1 p24抗原水平,采用荧光定量PCR(RT-PCR)测定两组细胞中的HIV-1 mRNA水平及TLRs通路上游因子mRNA水平。结果实验组MT2细胞HIV-1 mRNA的表达水平显著低于对照组,细胞培养上清液中的p24抗原水平显著低于对照组,差异有统计学意义(P<0.05)。实验组细胞的TLR3 mRNA水平显著高于对照组,差异有统计学意义(P<0.05);两组细胞的TLR2 mRNA、TLR8 mRNA、TLR9 mRNA水平比较差异无统计学意义(P>0.05)。实验组细胞的TLR3 mRNA、TRIF mRNA、IFN-β mRNA水平显著高于对照组,差异有统计学意义(P<0.05)。结论在MT2细胞中,瘦素可抑制HIV-1病毒的复制,且可上调TLR3/TRIF通路中TLR3、TRIF、IFN-β mRNA的表达水平。
Abstract:
ObjectiveTo investigate the effects of leptin on the replication of HIV-1 virus in MT2 cells and the related factors of TLR3/TRIF pathway. MethodsMT2 cells were cultured in vitro and divided into control group and experimental group in the logarithmic growth phase. The experimental group was cultured with 1 μg/mL leptin medium and the control group was cultured with conventional medium for 24h. The two groups were infected with HIV-1 3B virus respectively and cultured for 72h. The level of HIV-1 p24 antigen in cell culture supernatant was measured by enzyme-linked immunosorbent assay (ELISA). The level of HIV-1 mRNA and upstream factor mRNA of TLRs were measured by real-time PCR (RT-PCR). ResultsThe level of HIV-1 mRNA expression in the experimental group was significantly lower than that in the control group, the level of p24 antigen in the cell culture supernatant was significantly lower than that in the control group, the difference was statistically significant (P<0.05). The TLR3 mRNA level in the experimental group was significantly higher than that in the control group, the difference was statistically significant (P<0.05). There was no significant difference in TLR2 mRNA, TLR8 mRNA and TLR9 mRNA levels between the two groups (P>0.05). The TLR3 mRNA, TRIF mRNA and IFN-β mRNA levels in the experimental group were significantly higher than those in the control group, the difference was statistically significant (P<0.05). ConclusionLeptin can inhibit the replication of HIV-1 virus and up-regulate the mRNA expression of TLR3, TRIF and IFN-β in TLR3/TRIF pathway in MT2 cells.

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备注/Memo

备注/Memo:
▲基金项目:国家自然科学基金资助项目(31860040,81460511),广西生物医药协同创新中心研究生创新创业项目(GCICB-IE-2017012, GCICB-IE-2017013)
*通信作者:叶力,广西艾滋病防治研究重点实验室,电子邮箱 yeli@gxmu.edu.cn
更新日期/Last Update: 2019-05-09