[1]刘欣,叶力,蒋俊俊,等.苦豆碱的体外细胞毒性以及抗HIV-1活性实验研究▲[J].内科,2019,14(02):133-136.[doi:10.16121/j.cnki.cn45-1347/r.2019.02.03]
 LIU Xin,YE Li,JIANG Junjun,et al.Experimental study on cytotoxicity and anti-HIV-1 activity of aloperine in vitro[J].Internal Medicine of China,2019,14(02):133-136.[doi:10.16121/j.cnki.cn45-1347/r.2019.02.03]
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苦豆碱的体外细胞毒性以及抗HIV-1活性实验研究▲()
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《内科》[ISSN:1006-6977/CN:61-1281/TN]

卷:
14卷
期数:
2019年02
页码:
133-136
栏目:
论著
出版日期:
2019-04-30

文章信息/Info

Title:
Experimental study on cytotoxicity and anti-HIV-1 activity of aloperine in vitro
文章编号:
1673-7768(2019)02-0133-04
作者:
刘欣1叶力2蒋俊俊2刘洁1胡茜2周波2梁浩12*
1 广西医科大学生命科学研究院,南宁市530021;2 广西艾滋病防治研究重点实验室,南宁市530021
Author(s):
LIU Xin1 YE Li2 JIANG Junjun2 LIU Jie1 HU Xi2 ZHOU Bo2 LIANG Hao12
1 Life Sciences Institute, Guangxi Medical University; 2 Guangxi Key Laboratory of AIDS Prevention and Treatment, Nanning 530021, China
关键词:
苦豆碱细胞毒性抗HIV-1活性
Keywords:
Aloperine CytotoxicityAnti-HIV-1 activity
分类号:
R 965.1
DOI:
10.16121/j.cnki.cn45-1347/r.2019.02.03
文献标志码:
A
摘要:
目的探讨单体化合物苦豆碱的体外细胞毒性以及抗HIV-1活性作用,为寻找新的艾滋病治疗药物提供依据。方法以TZM-bl-HIV-1IIIB系统和MT-2-HIV-1IIIB系统为实验模型,用不同稀释度的苦豆碱溶液分别与TZM-bl细胞、MT-2细胞共同培养。采用ATP细胞活力检测试剂检测细胞的相对活力,观察苦豆碱对TZM-bl细胞、MT-2细胞的毒性作用;用HIV-1IIIB感染TZM-bl细胞和MT-2细胞,利用基于TZM-bl细胞的荧光素酶检测试剂,检测HIV病毒相对水平,观察苦豆碱抑制HIV-1活性的作用。结果在TZM-bl-HIV-1IIIB系统中,苦豆碱对TZM-bl细胞的半数细胞毒性浓度(CC50)>215.183 μM,苦豆碱对HIV-1IIIB的半数抑制浓度(IC50)为3.403 μM,治疗指数(TI)>63.2。在MT-2-HIV-1IIIB系统中,苦豆碱对MT-2细胞的CC50为802.000 μM,对HIV-1IIIB的IC50为47.190 μM, TI为>16.9。结论苦豆碱毒性小,抗HIV-1活性明显,具有潜在的开发利用价值。
Abstract:
ObjectiveTo observe the cytotoxicity and anti-HIV-1 activity effect of the monomeric compound aloperine and provide a basis for finding new drugs for AIDS treatment. MethodsThe TZM-bl-HIV-1IIIB system and the MT-2-HIV-1IIIB system were used as experimental models, the different concentrations of aloperine solution were used to co-culture with TZM-bl cells and MT-2 cells, respectively. The relative viability of cells was detected by ATP cell viability assay, and the toxicity of aloperine to TZM-bl cells and MT-2 cells was observed. HIV-1IIIB was used to infect TZM-bl cells and MT-2 cells, and the relative level of HIV-1 virus was detected by using the luciferase detection reagent based on TZM-bl cells, so as to observe the effect of aloperine on inhibiting the HIV-1 activity. ResultsIn the TZM-bl-HIV-1 IIIB system, the half cytotoxic concentration (CC50) of aloperine on TZM-bl cells was greater than 215.183 μM, the half inhibitory concentration (IC50) was 3.403 μM and the treatment index (TI) was greater than 63.2. In the MT-2-HIV-1 IIIB system, the CC50 of aloperine on MT-2 cells was 802.000 μM, the IC50 was 47.190 μM and the TI was greater than 16.9. ConclusionAloperine shows weak cytotoxicity and obvious anti-HIV-1 activity effect, it will be worthy of further study.

备注/Memo

备注/Memo:
▲基金项目:国家自然科学基金资助项目(8186130073),广西研究生教育创新计划项目(YCSW2018118) *通信作者:梁浩,广西医科大学生命科学研究院,电子邮箱 lianghao@gxmu.edu.cn
更新日期/Last Update: 2019-05-09