ObjectiveTo establish chromatogram-effect model based on inhibitory effect of four alkaloids (rhynchophyline, isorhynchophylline, corynoxine, corynoxine B) on oxidative stress of glutamate-induced PC12 cells. MethodsBased on the chromatogram-effect relation of inhibitory effect of four alkaloids on the oxidative stress of glutamate-induced PC12 cells, SPSS 15.0 was used to analyse data. ResultsOxidative stress of PC12 cells was significantly inhibited by rhynchophyline and corynoxine. The spectrum efficient equation was, Y=44.301+0.255X1 +0.356X3(P＜0.05).The deviation of predictive value and true value was no more than 10% by data validation from seven samples.ConclusionsThe chromatogram-effect model can be used to predict the effect of uncaria macrophylla wall against the oxidative stress of glutamate-induced PC12 cells.

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