ObjectiveTo explore the changes of Escherichia coli biofilm in medicine effects observed by optical microscopy along with silver staining method.MethodsEscherichia coli were cultivated in peritoneal dialysis solution-peritoneal dialysis cathete system to establish Escherichia coli biofilm model.After the biofilm was treated by medicine,we observe the changes of biofilm with optical microscope and scanning electronic microscope,respectively.ResultsAfter using silver staining method with the observation of optical microscope,the changes of Escherichia coli biofilm were showed no difference between optical microscope and scanning electronic microscope.ConclusionsThrough silver staining method,the changes of Escherichia coli biofilm can be more easily observed by optical microscope,and the experimental results were stable and reliable.

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