内科

目的探讨鸭流感病毒受体分布特点及其作用。方法应用凝集素组织化学染色技术检测鸭呼吸道和消化道流感病毒SA受体的分布，用荧光素Alexa 488标记禽流感病毒H9N1、人流感病毒H1N1，观察这两种病毒与鸭呼吸道、消化道各解剖部位结合特点。结果SAα-2，3Gal受体在鸭呼吸道气管、支气管、次级支气管、副支气管和消化道结肠呈高密度分布，而SAα-2，6Gal受体缺乏或仅极少量表达。禽流感病毒H9N1能与鸭呼吸道和消化道上皮细胞结合，而人流感病毒H1N1与副支气管和结肠未见结合反应，仅极少量与气管、支气管、次级支气管结合。结论水禽类鸭流感病毒SA受体的分布以SAα-2，3Gal受体为主，在呼吸道和消化道均呈高密度分布，有利于各亚型禽流感病毒在其复制、基因重配。

ObjectiveTo study the distribution characteristics and functions of influenza viruses SA(sialic acid,SA) receptors in ducks.MethodsThe distribution of SA receptors in respiratory tract and alimentary tract of ducks was investigated using lectin histochemical staining technology.Avian influenza H9N1 virus and human influenza H1N1 virus were labeled by Alexa 488,then their binding region of anatomy in respiratory tract and alimentary tract were observed respectively.ResultsThe SAα2,3Gal receptors were prevalent in the trachea,bronchus,second bronchus,parabrochus and colon of duck. On the contrary,the SAα2,6Gal receptors were deficient or infrequent.Furthermore,avian influenza H9N1 virus could bind to epithelial cells in respiratory tract and alimentary tract of duck.But human influenza H1N1 virus could not bind to the parabrochus and colon or bind infrequently to the trachea,bronchus,second bronchus of duck.ConclusionsThe SAα2,3Gal receptors were prevalent in respiratory tract and alimentary tract of duck.These results suggested that duck could provide an environment of replication and reassortment of avian influenza viruses.

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