ObjectiveTo search for an easy and cheap method for the culture of murine primary hepatocytes.MethodsThe murine liver was isolated by non-perfusion,digested by 0.2% IV-Collagenase so as to get the primary hepatocytes and culture them with DMEM medium in the monolayer.ResultsLiver cells were found under the optical microscope,showing as triangle,roundness,and so on.The cells was marshalling,and cell boundary was distinct.Cytoplasm was abundant and bright.There were one or two circular nucleuses in each cell.ConclusionThis method was good for the general labs to culture the hepatocytes,and it can provide a good basic for further research.

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