内科

目的探讨单味中药大黄和复方中药尿毒清对大鼠肾匀浆抗氧化应激作用的影响，并比较其效果，阐明中药抗氧化应激在治疗肾脏病中的作用。方法实验分组：空白组，模型组，维生素E组（阳性药物组），尿毒清组，大黄组（高、中、低三个浓度），去大黄的尿毒清组（高、中、低三个浓度）通过肾匀浆的自发脂质过氧化实验和Fe2+-H2O2诱导脂质过氧化实验，用硫代巴比妥酸（TBA）法测定各组在吸光度530 nm的OD值，计算脂质过氧化产物丙二醛（MDA）的抑制率。结果（1）尿毒清组OD值明显低于模型组，对肾匀浆自发性脂质过氧化的抑制率为61.56%，对肾匀浆Fe2+-H2O2诱导氧化的抑制率为57.43%，结果比较，差异有统计学意义（P＜0.05）；各浓度大黄和去大黄尿毒清OD值均低于模型组，抑制率均高于模型组，随浓度降低呈量效关系，结果比较，差异有统计学意义（P＜0.05）。（2）终质量浓度为0.65 mg/mL的尿毒清组、大黄组、去大黄尿毒清组与VitE组比较，尿毒清组、去大黄尿毒清组OD值低于VitE组，抑制率高于VitE组，结果比较，差异有统计学意义（P＜0.01）；VitE组OD值低于大黄组，抑制率高于大黄组，结果比较，差异有统计学意义（P＜0.01）。（3）同浓度的尿毒清、大黄和去大黄尿毒清比较，尿毒清组OD值均低于大黄组和去大黄尿毒清组，尿毒清组对肾匀浆自发氧化和Fe2+-H2O2诱导氧化的抑制率均高于大黄组和去大黄尿毒清组，结果比较，差异有统计学意义。结论（1）大黄及尿毒清可抑制大鼠肾匀浆脂质过氧化产物MDA的产生，具有明显的抗氧化应激作用。（2）复方中药尿毒清抗氧化应激作用效果优于单味中药大黄。（3）大黄浸膏和去大黄尿毒清高、中、低浓度组间抗氧化应激作用呈明显的量效关系。

ObjectiveTo explore the influences of rhubarb and Niaoduqing on anti-oxidative stress effects of kidney of rats and compare their effects,which further clarify the effect of using Chinese medicine on treating and preventing anti-oxidative stress of kidney disease.MethodsExperimental grouping:normal group,control group,positive drug group,Niaoduqing group,rhubarb groups of high,medium and low concentrations,Niaoduqing control groups (without rhubarb) of high,medium and low concentrations.Through Spontaneous lipid peroxidation and Fe2+- H2O2 induced lipid peroxidation experiments of kidney homogenate,using thiobarbituric acid (TBA) method to determinate the absorbance values of each group at OD 530nm,calculating the inhibition of lipid peroxidation MDA.Result(1) The OD value of Niaoduqing group was significantly lower than modeling group.The inhibitory rate of the spontaneous lipid peroxidation of kidney homogenate was 61.56% and the inhibitory rate of Fe2+-H2O2 induced lipid peroxidation of kidney homogenate was 57.43%.The results showed significantly different (P＜0.05).The OD values of rhubarb groups and Niaoduqing control groups were lower than modeling group.Concentration and lipid peroxidation products MDA formation was negative. Concentration effect of oxidative stress between the groups showed a dose-effect relationship,the results were significantly different (P＜0.05).(2) Compared final concentrations of 0.65 mg/mL of Niaoduqing group,rhubarb group and Niaoduqing control groups with VitE group,the OD value of Niaoduqing group and Niaoduqing control groups were lower than VitE group,the inhibitory rates were higher than VitE group.The results showed significantly different (P＜0.01). The OD value of VitE group was lower than rhubarb groups,and the inhibitory rate was higher than rhubarb group.The results showed significantly different (P＜0.01).(3)Compared of Niaoduqing,rhubarb and Niaoduqing control groups,the OD values of Niaoduqing group were lower than rhubarb groups and Niaoduqing control groups with the same concentration.Compared of the inhibitory rates of spontaneous lipid peroxidation and Fe2+-H2O2 induced lipid peroxidation of kidney homogenate,Niaoduqing group was higher than rhubarb groups and Niaoduqing control groups.The results were significantly different.ConclusionRhubarb and Niaoduqing have significant inhibition on the producing of MDA induced by peroxidation of rat kidney.The results also proved that the compound Chinese herbal Niaoduqing has better therapeutic effects than single Chinese herbal rhubarb on anti-oxidative stress.Concentration effect of oxidative stress between the groups of rhubarb and Niaoduqing control groups (without rhubarb) showed a dose-effect relationship.