ObjectiveTo screen agents with strong antifungal activity in soil,so as to provide basis for the discovery of material with antifungal activity.MethodsBacterial strains with strong antifungal activity were collected from Daming mountain,then were identified by molecular biology methods.ResultsOne strain JK16 with strong inhibition to Mucor was obtained from the sample.The 16s molecular identify test showed that JK16 strain shared most similarity with Burkholderia sp.m35b.So,it is named as Burkholderia cenocepacia JK16.ConclusionsJK16 strain has fungistasis activity.It is a potential producer of fungicidal antibiotic.Further study of metabolite produced by this strain is really needed.