Objective To screen out urine podocyte biomarkers with clinical value in the diagnosis of immunoglobulin A nephropathy (IgAN), and analyze their correlations with clinicopathological features. Methods A total of 129 IgAN patients were selected as the IgAN group, 60 idiopathic membranous nephropathy (IMN) patients as the IMN group, and 63 healthy volunteers as the healthy group. The urine levels of c‑Maf inducing protein(CMIP), Ras homolog gene family member A (RhoA), cofilin‑1, soluble urokinase‑type plasminogen activator receptor (suPAR), ɑ‑Klotho, nephrin, and podocalyxin (PCX) in the above three groups were detected and compared. The clinical value of urine podocyte biomarkers in the diagnosis of IgAN was analyzed using receiver operating characteristic (ROC) curve, and the correlations between urine podocyte biomarkers and clinical markers of IgAN patients were analyzed by the Pearson or Spearman correlation. Results There were differences in the levels of cofilin-1 and suPAR between IgAN and IMN groups and between IgAN and healthy groups(all P<0.05). The area under the ROC curve of suPAR in the diagnosis of IgAN was 0.730, and the area under the ROC curve of the combination of suPAR and cofilin-1 in the diagnosis of IgAN was 0.772 (all P<0.05). The results of correlation analysis showed that the suPAR level was positively correlated with systolic blood pressure, 24-hour urine protein, total cholesterol, high-density lipoprotein levels, and Oxford classification system score of endocapillary hypercellularity, and negatively correlated with the albumin level; the cofilin-1 level was positively correlated with both systolic and diastolic blood pressure, and negatively correlated with the estimated glomerular filtration rate. Conclusion The urine levels of podocyte biomarkers, suPAR and cofilin-1, are abnormally elevated in patients with IgAN, and the combination of suPAR and cofilin-1 levels has certain value in the non-invasive diagnosis of IgAN.