ObjectiveTo investigate the effect of acupoint injection Puerarin on ropivacaine infusion induced cardiac toxicity in rats and explore its mechanism. MethodsA total of 40 SD adult male rats were randomly divided into four groups, non-treatment group(group A), model group (group B), Puerarin prevention group (group C), Puerarin treatment group(group D) (n=10).Establish model of rats by intravenous injection 0.5% ropivacaine at a rate of 2mg/(kg·min). Group A was injected with saline in bilateral Neiguan point, group B and group D were injected with saline and Puerarin injection in bilateral Neiguan point when arrhythmia occurs; 30 minutes before pump into ropivacaine, group C was injected with Puerarin injection in bilateral Neiguan point. Heart rate(HR) before administration, and the time of arrhythmia(as QRS extend to 90 ms) and cardiac arrest emergence were recorded, the amount of ropivacaine was calculated, and the content of Na+-K+-ATPase and LDH of myocardium in rats was tested. ResultsThe time of arrhythmia and cardiac arrest emergence in group C and group D were significantly later than those in group B,and the amount of ropivacaine was significantly larger than that in group B (P＜0.05); compared with group B, the content of Na+-K+-ATPase of myocardium in group C and group D was significantly increased and the content of LDH was significantly decreased (P＜0.05). ConclusionsAcupoint injection Puerarin has the effects of prevention and treatment on the cardiac toxicity induced by ropivacaine infusion, the protection mechanism may be due to increase the content of Na+-K+-ATPase of myocardial and reduce the content of LDH.

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