内科

目的探讨miR-210基因和KCMF1蛋白在先兆子痫（PE）患者胎盘中的表达水平及临床意义。方法选取14例重度PE患者、27例轻度PE患者、12例正常孕妇为研究对象，分别检测比较她们的血压、尿蛋白（UPC）、体重指数（BMI）以及胎盘中miR-210和KCMF1表达情况，对miR-210与KCMF1表达的关系、miR-210和KCMF1的表达分别与血压、UPC水平的关系进行相关性分析。结果轻度PE组患者SBP、DBP、UPC水平显著高于正常对照组(P＜0.05），重度PE组患者SBP、DBP、UPC水平显著高于轻度PE组(P＜0.05）。轻度PE患者胎盘中miR-210的表达水平显著高于正常对照组、KCMF1表达水平显著低于正常对照组(P＜0.05）；重度PE组患者胎盘中miR-210的表达水平显著高于轻度PE组、KCMF1表达水平显著低于轻度PE组(P＜0.05）。轻度、重度PE组患者胎盘中miR-210和KCMF1的表达水平均呈负相关(P＜0.05）；轻度、重度PE组患者胎盘中miR-210的表达和SBP、DBP、UPC水平均呈正相关(P＜0.05），KCMF1表达和SBP、DBP、UPC水平均呈负相关(P＜0.05）。结论PE患者胎盘中miR-210表达上调，KCMF1表达下调，miR-210可能通过调控KCMF1的表达水平进而影响PE的发生发展。

ObjectiveTo investigate expression and significance of miR-210 gene and KCMF1 protein in placenta of preeclampsia patients. Methods14 cases of severe PE, 27 cases of mild PE, and 12 normal pregnant women were selected as the subjects, whose blood pressure, urine protein (UPC), body mass index (BMI), and the expression of miR-210 and KCMF1 in the placenta were measured and compared respectively. The relationship between the expression of miR-210 and KCMF1, the expression of miR-210 and KCMF1 and the relationship between the blood pressure and the level of UPC were analyzed. ResultsThe levels of SBP, DBP and UPC in the mild PE group were higher than those in the normal control group (P＜0.05). The levels of SBP, DBP and UPC in the severe PE group were higher than those in the mild PE group (P＜0.05). The expression level of miR-210 in placenta of mild PE patients was higher than that in normal control group, and the expression level of KCMF1 was lower than that of normal control group (P＜0.05). The expression level of miR-210 in the placenta of severe PE group was higher than that in mild PE group, and the expression level of KCMF1 was lower than that in mild PE group (P＜0.05). The expression level of miR-210 and KCMF1 in the placenta of mild and severe PE group was negatively correlated (P＜0.05). The expression of miR-210 in the placenta of mild and severe PE group was positively correlated with the level of SBP, DBP and UPC, and the expression of KCMF1 was negatively correlated with the level of SBP, DBP and UPC. ConclusionThe expression of miR-210 in the placenta of PE patients was up-regulated, and the expression of KCMF1 was down regulated. MiR-210 may affect the development of PE by regulating the expression level of KCMF1.