ObjectiveTo investigate the effects of high-fat diet combined with vitamin intraperitoneal injection D3 on the expression of AU-rich element RNA-Binding factor 1 (AUF1) and interleukin 35 (IL-35) in rat arterial tissue. Methods20 SD rats were randomly divided into experimental group and control group. The experimental group were given a high-fat diet and intraperitoneal injection of vitamin D3. The control group was raised normally. After 12 weeks, the blood lipid levels and arterial tissue lesions were compared between the two groups. The mRNA and protein expressions of AUF1 and IL-35 were compared between the two groups. ResultsThe serum total cholesterol and triglyceride in the experimental group were significantly higher than those in the control group, whose the expression of AUF1 mRNA and IL-35 mRNA in the arterial tissue was significantly higher than that in the control group (P＜0.05). Under light microscope, the arterial intima of the control group was smooth, the smooth muscle cells of the middle membrane were arranged neatly, the fibers were clear, the boundary was clear, and there was no infiltration of inflammatory cells. In the experimental group, the aortic endothelial cells of the experimental group were swollen and thick, and the smooth muscle cells of the medium were disordered. There was a slight local proliferation, but no plaque tissue. There were no obvious bands in the protein blots of AUF1 and IL-35 in the arteries of the two groups. ConclusionThe high-fat diet combined with intraperitoneal injection of vitamin D can induce a certain degree of inflammatory reaction in rat arteries, which has a significant effect on the expression of AUF1 mRNA and IL-35 mRNA in arterial tissue. However, it did not have a significant effect on the expression of IL-35 and AUF1 protein in arterial tissue, which may be related to the shorter experimental period of the rats in this study. AUF1 and IL-35 can be used as targets for anti-inflammatory response.

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