ObjectiveTo investigate the effects of leptin on the replication of HIV-1 virus in MT2 cells and the related factors of TLR3/TRIF pathway. MethodsMT2 cells were cultured in vitro and divided into control group and experimental group in the logarithmic growth phase. The experimental group was cultured with 1 μg/mL leptin medium and the control group was cultured with conventional medium for 24h. The two groups were infected with HIV-1 3B virus respectively and cultured for 72h. The level of HIV-1 p24 antigen in cell culture supernatant was measured by enzyme-linked immunosorbent assay (ELISA). The level of HIV-1 mRNA and upstream factor mRNA of TLRs were measured by real-time PCR (RT-PCR). ResultsThe level of HIV-1 mRNA expression in the experimental group was significantly lower than that in the control group, the level of p24 antigen in the cell culture supernatant was significantly lower than that in the control group, the difference was statistically significant (P＜0.05). The TLR3 mRNA level in the experimental group was significantly higher than that in the control group, the difference was statistically significant (P＜0.05). There was no significant difference in TLR2 mRNA, TLR8 mRNA and TLR9 mRNA levels between the two groups (P＞0.05). The TLR3 mRNA, TRIF mRNA and IFN-β mRNA levels in the experimental group were significantly higher than those in the control group, the difference was statistically significant (P＜0.05). ConclusionLeptin can inhibit the replication of HIV-1 virus and up-regulate the mRNA expression of TLR3, TRIF and IFN-β in TLR3/TRIF pathway in MT2 cells.

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