ObjectiveTo investigate the value of serum anti-Mullerian hormone (AMH), estradiol (E2), luteinizing hormone (LH), and follicle stimulating hormone (FSH) levels in evaluating the ovarian function of female infertile patients. MethodsA total of 150 female infertilities admitted to the gynecology department of our hospital from January to June 2019 were selected as observation group, and 150 parous healthy women underwent health examinations in our hospital during the same time were selected as control group. The E2, LH, FSH levels, and the three-level-combination receiver operating characteristic (ROC) curves predicting infertilities were drawn to evaluate its value of ovarian function in predicting infertilities. ResultsThere was no statistically significant difference of serum AMH level between the two groups (P>0.05). The levels of serum E2, LH and FSH in the control group were significantly higher than those in the observation group, and the differences were statistically significant (P<0.05). The area under the curve (AUC) for E2, LH, FSH, and the three-level combination predicting infertilities were 0.897, 0.938, 0.902, and 0.980, respectively. ConclusionThe ovarian reserve function of infertilities is impaired. The detection of E2, LH and FSH combination in predicting infertility has the highest sensitivity and specificity, which can better reflect the ovarian function in female infertilities.