内科

目的观察八卦头针疗法对APP/PS1双转基因小鼠行为学及β-淀粉样蛋白（Aβ）表达的影响，探讨八卦头针治疗阿尔茨海默病（AD）可能的作用机制。方法将30只9月龄的APP/PS1双转基因小鼠（AD小鼠）随机分为模型组、八卦头针组、多奈哌齐组，把同品系同月龄C57BL/6J小鼠（正常小鼠）10只设为对照组。给予对照组、模型组、八卦头针组小鼠生理盐水灌胃，同时给予八卦头针组小鼠八卦头针干预，给予多奈哌齐组小鼠多奈哌齐灌胃，干预4周。比较4组小鼠的空间学习记忆能力；检测比较4组小鼠海马区Aβ1-40和Aβ1-42蛋白水平。结果定位航行实验第1天至第5天，模型组小鼠的逃避潜伏期显著长于对照组（P＜0.05）；第2天至第5天多奈哌齐组和八卦头针组小鼠的逃避潜伏期显著短于模型组，差异有统计学意义（P＜0.05）。模型组小鼠的穿越平台次数、在目标象限游泳时间显著少（短）于对照组（P＜0.05）；多奈哌齐组和八卦头针组小鼠的穿越平台次数、在目标象限游泳时间显著多（长）于模型组，差异有统计学意义（P＜0.05）。模型组小鼠海马区的Aβ1-40、Aβ1-42蛋白表达水平明显高于对照组，八卦头针组、多奈哌齐组小鼠海马区的Aβ1-40、Aβ1-42蛋白表达水平显著低于模型组，差异有统计学意义（P＜0.05）。结论八卦头针可明显改善阿尔茨海默病小鼠的学习记忆障碍，可能与其降低了AD小鼠海马区Aβ1-40、Aβ1-42蛋白水平有关。

ObjectiveTo observe the effect of Bagua scalp acupuncture on the behavioristics and amyloid β-protein (Aβ) expression of APP/PS1 double transgenic mice, and to explore the possible mechanism of Bagua scalp acupuncture in the treatment of Alzheimer disease (AD). MethodsThirty 9-month-old APP/PS1 double transgenic mice (AD mice) were randomly divided into model group, Bagua scalp acupuncture group, and donepezil group, as well as 10 C57BL/6J mice (normal mice) in the same strain and the same-month-old were assigned to control group. Gastric perfusion was given to the mice in the control, model and Bagua scalp acupuncture groups; meanwhile, mice in the Bagua scalp acupuncture group were intervened with Bagua scalp acupuncture, and the mice in the donepezil group were given gastric perfusion of donepezil. All mice were intervened for 4 weeks. The spatial learning and memory ability of the 4 groups of mice were compared; the protein levels of Aβ1-40 and Aβ1-42 in the hippocampus were detected and compared between the 4 groups. ResultsFrom the first to the fifth day of the place navigation, the escape latency of mice in the model group was significantly longer than that in the control group (P＜0.05); the escape latency of the mice in the donepezil group and the Bagua scalp acupuncture group from the second to the fifth day was significantly shorter than that in the model group, and the difference was statistically significant (P＜0.05). The number of crossing platform and swimming time in the target quadrant of the model group were significantly less (shorter) than those of the control group (P＜0.05), whereas the number of crossing platform and swimming time in the target quadrant of the mice in the donepezil group and the Bagua scalp acupuncture group were significantly more (longer) than those in the model group, with statistically significant differences (P＜0.05). The protein expression levels of Aβ1-40 and Aβ1-42 in the hippocampus of the model group were significantly higher than those of the control group, whereas the expressions mentioned above of the Bagua scalp acupuncture group and donepezil group were significantly lower than those of the model group, and the differences were statistically significant (P＜0.05). ConclusionBagua scalp acupuncture can significantly improve the learning and memory impairment of AD mice, which may be related to the decrease of Aβ1-40 and Aβ1-42 protein levels in the hippocampus of AD mice.