ObjectiveTo establish bleomycin-induced mice model of scleroderma, to observe and analyze the pathological characteristics of intestinal complications of scleroderma, and to provide a reference for in-depth study on intestinal complications of scleroderma by establishing a favourable animal model. MethodsA total of 20 female BALB/c mice were selected and divided into control group and model group by the random number method, with 10 mice in each group. The mice in the control group were subcutaneously injected with 0.1 mL of phosphate-buffered saline (PBS) on the back, once a day, whereas the mice in the model group were injected subcutaneously with 0.1 mL of bleomycin PBS on the back to establish a scleroderma model, once a day. Both groups of mice were injected continuously for 4 weeks. On the second day after establishing the model, the mice received fasting but no water-depriving. After 24 hours, the mice were intragastrically administrated with semi-solid nutritional paste and then the mice were killed by neck removal after 20 minutes of gavage. The pathological characteristics of the skin of injection area on the back, lung and colon tissues biopsies of the two groups were observed and compared. The small intestine propulsion rate was compared between the two groups. ResultsThe skin, lung and colon tissues of the model group had fibrosis and inflammatory infiltration changes. The small intestine propulsion rate of the model group was lower than that of the control group, with statistically significant differences (P＜0.05). ConclusionThe bleomycin-induced scleroderma mice model has obvious intestinal pathological changes, which can be used as an ideal model to study the pathogenesis of intestinal complications of scleroderma.

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