ObjectiveTo investigate the expression level of TLR4 protein and the polymorphism of rs4986790A/G and rs4986791C/T loca in Zhuang nationality patients with coronary heart disease (CHD). MethodsA total of 245 Zhuang nationality patients who were confirmed with CHD by coronary angiography (CAG) in our hospital from January 2016 to January 2018 were selected as the CHD group, and 245 non-CHD patients who were hospitalized in our hospital during the same period were selected as the control group. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum TLR4 protein expression levels of the two groups. The SNaPshot sequencing method was used to detect the genotypes and alleles of the rs4986790A/G and rs4986791C/T loca of the TLR4 gene in peripheral blood of the two groups. The correlation between TLR4 protein expression level, the polymorphisms of rs4986790A/G and rs4986791C/T loca and the incidence of CHD in the Zhuang population was analyzed. ResultsThe expression level of TLR4 protein in peripheral blood in the CHD group was higher than that in the control group, with a statistically significant difference (P＜0.05). There were no statistically significant differences in the frequency distribution of alleles and genotypes at the rs4986790A/G locus and the rs4986791C/T locus between the CHD group and the control group (P＞0.05). ConclusionThe expression level of TLR4 protein in peripheral blood is correlated with the occurrence of CHD in Guangxi Zhuang population, but the polymorphisms of TLR4 gene in rs4986790A/G and rs4986791C/T loca may not be significantly related to the incidence of CHD in Zhuang population.

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