ObjectiveTo investigate the relationship between sperm DNA fragmentation index (DFI) and sperm conventional parameters, sperm acrosin activity in infertility patients with prolonged liquefaction and its effect on in vitro fertilization (IVF) outcome. MethodsA total of 106 infertility patients with prolonged liquefaction in our hospital from December 2018 to May 2020 were selected as the observation group. And 52 male volunteers with normal healthy fertility history who underwent physical examination in our hospital during the same period were selected as the control group. Semen parameters (including semen concentration, normal sperm morphological rate, progressive sperm quantity), sperm acrosin activity, sperm DFI, smoking and lifestyle condition were compared between the two groups. Multivariate logistic regression analysis was performed on the factors affecting the sperm DFI of infertility patients with prolonged liquefaction. Pearson correlation analysis was performed on the relationship between sperm DFI, sperm acrosin activity and age, semen parameters in prolonged liquefaction infertility patients. The IVF outcomes of prolonged liquefaction patients with different sperm DFI were compared. ResultsThe semen concentration, normal sperm morphology rate, progressive sperm quantity and sperm acrosin activity in the observation group were significantly lower than those in the control group, while sperm DFI, smoking rate and the incidence of irregular lifestyle were significantly higher than those in the control group, with statistically significant differences (P＜0.05). Multivariate logistic regression analysis showed that age, semen concentration, normal morphology rate, progressive sperm quantity, sperm acrosin activity, smoking and irregular daily routine were all independent factors affecting sperm DFI of infertility patients with prolonged liquefaction. Pearson correlation analysis showed that the sperm DFI of infertility patients with prolonged liquefacient was positively correlated with age, and was negatively correlated with semen concentration, normal sperm morphology rate, progressive sperm quantity and sperm acrosin activity. Sperm acrosin activity in infertility patients with prolonged liquefaction was negatively correlated with age, and was positively correlated with the semen concentration, normal sperm morphology rate, progressive sperm quantity. The normal fertilization rate of IVF in prolonged liquefaction patients with sperm DFI＜10% was significantly higher than that in patients with sperm DFI≥10% (P＜0.05), but there was no statistically significant difference in their cleavage rate of embryos (P＞0.05). ConclusionThe sperm DNA fragmentation index of infertility patients with prolonged liquefaction is closely related to their sperm conventional parameters and sperm acrosin activity, and the sperm DNA fragmentation index may have an important influence on the outcomes of in vitro fertilization.

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