内科

目的研究柔红霉素（Daunorubicin，DNR）对人急性早幼粒细胞白血病（APL）细胞株HL-60细胞中干扰素调节因子1（IRF1）-mRNA、干扰素调节因子8（IRF8）-mRNA、干扰素调节因子9（IRF9）-mRNA表达的影响。方法将HL-60细胞株设为HL-60组、HL-60+DNR组，同时取3例正常人外周血白细胞为NC组。HL-60组为未加药处理组，HL-60+DNR组为小剂量DNR持续作用HL-60细胞10 d。采用实时荧光定量聚合酶链反应（RT-PCR）法检测IRF1-mRNA、IRF8-mRNA、IRF9-mRNA转录水平，每组实验重复3次。结果与NC组相比，IRF1-mRNA、IRF8-mRNA转录水平在HL-60组显著下调（P＜0.05），而HL-60+DNR组显著上调（P＜0.05）；与NC组相比，IRF9-mRNA转录水平在HL-60+DNR显著上调（P＜0.05），在HL-60组则表现为下调，但无统计学差异（P＞0.05）。结论DNR可上调HL-60细胞中的IRF1、IRF8、IRF9表达水平。APL经DNR治疗后，可能通过上调IRF1、IRF8、IRF9的表达诱导白血病细胞的凋亡，促进其成熟，促进病情的缓解。

ObjectiveTo explore the effect of daunorubicin(DNR) on the expression of IRF1-mRNA, IRF8-mRNA and IRF9-mRNA in acute promyelocytic leukemia cell line of HL-60 cell. MethodsHL-60 cell line was set to HL-60 group, HL-60+DNR group, and the peripheral white blood cells separated from the blood of normal person served as NC group. The HL-60+DNR group was exposed to small doses of DNR every two day for 10 days, while the HL-60 group received no treatment. IRF1-mRNA,IRF8-mRNA and IRF9-mRNA were detected by real-time reverse transcription polymerase chain reaction(RT-PCR). The analysis for each group was performed for three times. ResultsThe expression of IRF1-mRNA and IRF8-mRNA in the HL-60 group were markedly down-regulated compared to the NC group(P＜0.05), but were markedly up-regulated in the HL-60+DNR group(P＜0.05); compared to the NC group, the expression of IRF9-mRNA in the HL-60+DNR group was markedly up-regulated (P＜0.05), and down-regulated in the HL-60 group, but there was no significant difference between the two groups (P＞0.05) .ConclusionsDNR can up-regulate the expression of IRF1、IRF8、IRF9 in HL-60 cell line. DNR may induce apoptosis and promote maturation on leukemia cells and promote remission in APL patients.