ObjectiveTo construct the eukaryotic expression vector of human Tie2 and establish its stable transfected SKOV3 cell line. MethodsThe CDS of Tie2 was amplified from BEL-7402 cell line by RT-PCR and cloned into pEGFP-N1. The recombinant plasmid pEGFP-N1-Tie2 was sequenced. SKOV3 cells were transfected with pEGFP-N1-Tie2 and selected with G418 and than isolated monoclonal. The expression of Tie2 was identified by q-PCR and Western Blot. ResultsThe eukaryotic expressing plasmid of pEGFP-N1-Tie2 was successfully constructed and Tie2 stably expressing SKOV3 cell line was established.ConclusionsThe recombinant eukaryotic expression vector pEGFP-N1-Tie2 and the established SKOV3 cell line stably expressing Tie2 may be used for further study of Tie2 function.

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