ObjectiveTo investigate the expression of CD95 on CD8+ T cells by test result and find out the relationship between the expression of CD95 and apotosis of CD8+T cells in chronic hepatitis B patients. MethodsPeripheral blood was collected from 37 chronic hepatitis B patients, and density gradient centrifugation was used to isolate PBMCs from peripheral blood. Flow cytometry was employed to test the CD95 expression of CD8+T cells in chronic hepatitis B patients. ResultsCD95 expression of CD8+T cells in both high viral load group and low viral load group were significantly higher than that in the healthy group (P＜0.05), moreover, the expression of CD95 was negatively related to numbers of CD8+T cell (r=-0.3486) . ConclusionsThe high expression of CD95 promotes apoptosis of CD8+T cells and weakens CD8+T cell′s antiviral effect, which may be associated with the formation of chronic Hepatitis B and the persistent infection of HBV.

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